Degradation and DT-50 of Acenaphthylene in River Sediment under Ambient Conditions and Effect of Nutrients: An HPLC and GLC Analysis

IJEP 42(3): 374-379 : Vol. 42 Issue. 3 (March 2022)

Abdul Kaabi, Apu Das and Prahash Chandra Sarma*

Cotton University, Department of Chemistry, Guwahati – 781 001, Assam, India


The degradation of acenaphthylene in river sediment was investigated in a 60 days experiment under two different chemical environments. GLC and HPLC analyses of soxhlet extracts in DCM and methanol solvent, respectively were done to find out the concentration of remaining hydrocarbon. By applying the method of integration on the results on concentration as per HPLC analysis, the rate of the chemical process was found to follow first-order kinetics. Disappearance time for 50% in days, that is DT-50 of the PAH was determined by using half-life equation of first-order reaction. It has been found that the DT-50 value under ambient conditions was found to be 41 days. Gas liquid chromatography was done for 53 min of two samples. The GLC report of first 10 min analysis exhibit that the total number of n-hexane soluble compounds in the acenaphthylene applied to soil decreases from 30 to 23 under the influence of NPK nutrients. These 23 peaks cover a total area of 96.765% in the chromatogram against 98.161% by the 30 peaks of the sample without NPK nutrients. This indicates that the presence of NPK nutrients accelerates the removal of the hydrocarbon and its derivatives from the soil.


DT-50, Acenaphthylene, Degradation, River sediment, NPK nutrients


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