Bioremediation of Acetaminophen Mediated by Klebsiella pneumoniae subsp. pneumoniae DSM 30104 (T)

IJEP 43(7): 579-587 : Vol. 43 Issue. 7 (July 2023)

Meghmala Waghmode1, Neha Patil1* and Aparna Gunjal2

1. PDEA’s Annasaheb Magar Mahavidyalaya, Department of Microbiology, Pune, Maharashtra – 411 028, India
2. Dr. D. Y. Patil Arts, Commerce and Science College, Department of Microbiology, Pune, Maharashtra – 411 018, India


Acetaminophen is one of the widely used over-the-counter drugs. Its residual concentration has been detected in pharmaceutical industry wastewater, effluent treatment plant and surface water. Eco-toxicological effects of paracetamol have been reported on seed germination, fish and algae. Drug resistance in pathogenic micro-organisms has been attributed to the overuse or continuous exposure of pathogens to drugs. The work was conducted from February 2022 to July 2022. Current study was carried out on the acetaminophen (paracetamol) degradation potential of Klebsiella pneumoniae subsp. pneumoniae DSM 30104(T) which was isolated from effluent of pharma industry. Identification was done using 16S rRNA sequencing technique. Isolate was found to degrade 82.8% of acetaminophen (2500 ppm) after five days of incubation. Based on the analytical techniques high resolution mass spectrometry (HRMS) and proton nuclear magnetic spectroscopy (1H NMR) study, 4-aminophenol was found to be biodegradative metabolite. Klebsiella pneumoniae subsp. pneumoniae DSM 30104(T) can degrade paracetamol through biological approach. Compared to paracetamol, its biodegradative product 4-aminophenol has more toxicity against algae. The paracetamol biodegradation potential of Klebsiella pneumoniae subsp. pneumoniae DSM 30104(T) was studied and could be used as microbial candidate for remediation of paracetamol contaminated sites.


Acetaminophen, Biodegradation, Pathogens, Phyloge-netics, Wastewater


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