Isolation, Identification and Characterization of Endophytic Bacteria Isolated from Root Nodules of Leguminous Clover Plant Species

IJEP 44(13): 1153-1161 : Vol. 44 Issue. 13 (Conference 2024)

Soni Singh, Vipin Parkash* and Diksha

Indian Council of Forestry Research and Education (ICFRE), Forest Research Institute, Forest Protection Discipline, Dehradun – 248 006, Uttarakhand, India

Abstract

The objective of the study is to assess the potential of endophytic bacteria within Trifolium species that can contribute to sustainable agroforestry/agriculture and land management practices. By reducing the reliance on synthetic fertilizers and promoting soil health, these practices can play a vital role in controlling pollution, particularly in mitigating nitrogen-related pollution and minimizing environmental impact of conventional land management practices. Plant nodule samples were collected from cultivated fields in Sudhowala, Prem Nagar and Dehradun. Trifolium alexandrinum L. had two types of root nodules, type I (off-white) in primary and lateral roots and type II (brown) exclusively in lateral roots. Trifolium resupinatum L. had uniform white nodules in both primary and lateral roots. Stereomicroscopy showed nodules scattered throughout the root system. Three gram-negative strains of endophytic bacteria were isolated, not absorbing Congo Red in the yeast extract mannitol agar (YEMA) culture. Despite morphological differences, both Trifolium alexandrinum nodule types exhibited similar biochemical traits, indicating potential microhabitat variations in root system. This enhances nitrogen acquisition across diverse conditions, boosting adaptability. The isolated strains were confirmed as endophytic rhizobacteria. Trifolium alexandrinum L. had higher leg haemoglobin concentrations (0.79 mµ and 0.39 mµ) than Trifolium resupinatum L. (0.47 mµ) at 540 nm, emphasizing the role of leg haemoglobin in nitrogen fixation.

Keywords

Leg haemoglobin, Nitrogen fixation, Rhizobacteria, Silvopastoral systems

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