IJEP 44(14): 1268-1276 : Vol. 44 Issue. 14 (Conference 2024)
1. Government Engineering College -Valsad, Chemical Engineering Department, Valsad – 396 001, Gujarat, India
2. Harcourt Butler Technical University, Department of Chemical Engineering, Kanpur – 208 002, Uttar Pradesh, India
3. UPL University of Sustainable Technology, Chemical Engineering Department, Shroff S. R. Rotary Institute of Chemical Technology, Ankleshwar – 393 135, Gujarat, India
4. Gujarat Technological University, Chandkheda, Ahmedabad – 382 424, Gujarat, India
Abstract
Steviol glycoside (SGs) is a well-known, intense natural sweetener and non-caloric sweetener. Its widespread application requires an innovative extraction, isolation and purification method to obtain steviol glycosides (SGs) in crystal form. This study sought to improve the extraction step by applying response surface methodology (RSM). A Box-Behnken design (BBD) was applied under these independent parameters: different solid-to-liquid ratio, S/L (10, 15 and 20) water as a solvent for different times (1 hr, 3 hr and 5 hr) at different temperatures (40oC, 60oC and 80oC). In our work, the determination coefficient R2 was 96.73%, which shows that experimental data were satisfactory. It was discovered that the percentage of extraction recovery of steviol glycosides from Stevia leaves could be at a maximum of 51.09% at the optimum condition at an immersion temperature of 80°C, S/L=10 and an extraction time of 5 hr. 47.87% was the experimental extraction recovery obtained from Stevia extraction at the feasible optimum condition. This work can be used to maximize the recovery from the extraction step, followed by the isolation steps, which can be further scaled up.
Keywords
Steviol glycosides, Stevioside, Rebaudiana-A, Stevia rebaudiana Bertoni, Response surface methodology, Box-Behnken design
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