Green Synthesis of Silver Nanoparticles of Ixora coccinea L. Leaf Extract and Its Antibacterial Activity

Silver nanoparticles have gained popularity due to their diverse pharmacological actions, like anti-inflammatory, antibacterial, anticancer action, etc. Green synthesis of silver nanoparticles incorporates medicinal plant extract and silver nitrate. Ixora coccinea is a commonly grown medicinal plant in Gujarat and many parts of India. I. coccinea exhibited many medicinal properties, such as anti-inflammatory, hepatoprotective and anticancer properties. In the present research, a silver nanoparticle of I. coccinea leaf water extract was prepared using the green synthesis method. In-vitro antibacterial activity of the synthesized nanoparticles was evaluated against Pseudomonas aeruginosa and Staphylococcus aureus using the disc diffusion method. Synthesized nanoparticles were subjected to evaluation for ultraviolet spectroscopy, infrared spectroscopy and particle size measurement using a zeta sizer. The results showed that synthesized nanoparticles showed characteristic absorbance maxima around 412-413 nm. Infra-red spectroscopy showed stretching at 1749/cm, alkene stretching at 1427.1/cm and alkyl stretching around 2919/cm. The synthesized nanoparticles showed a diameter ranging from 91-94 nm, which confirms that the particles are at nano size. In the case of in-vitro antibacterial activity, nanoparticles showed a zone of inhibition of 20.25±1.75 mm and 16.5±1.5 mm against P. aeruginosa and S. aureus, respectively.