Hydrogen production from axenic isolated cultures and consortia of Clostridium thiosulphatireducens and Enterobacter aerogenes on soybean straw

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IJEP 45(2): 103-113 : Vol. 45 Issue. 2 (February 2025)

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Pradnya Gautam Kedar1, Aparna Nair2, Ram Ashok Konale1 and Sopan Tukaram Ingle1*

1. KBC North Maharashtra University, School of Environmental and Earth Sciences, Jalgaon – 425 001, Maharashtra, India
2. Curo Biosciences Pvt. Ltd., Nagpur – 440 020, Maharashtra, India

Abstract

In this study, biohydrogen production from soybean straw in an anaerobic batch reactor (sera bottles) using H2-producing bacteria (Clostridium thiosulphatireducens and Enterobacter aerogenes) was investigated. Candidate strains were identified and analyzed by phylogenetic analysis. These bacteria were tested for their biohydrogen production individually as well as in combination. C. thiosulphatireducens, E. aerogenes and their co-culture inoculums were named as strain I, strain II and co-culture inoculum, respectively. The fermentation process was carried out at 37°C at pH 6. Physico-chemical characteristics of substrate and 16S rDNA gene sequences were investigated. The maximum biohydrogen yield obtained was 1.39 mol of H2/g TS. Byproducts formed during fermentation were acetic, butyric, propionic and formic acid. Biohydrogen has drawn the attention of researchers all over the world due to its advantages over conventional fuels. While highly promising as a biofuel, the process of biohydrogen production is still not economically viable and environmentally sustainable. Advances in improvements of hydrogen yield through sustainable means are urgently needed through process modifications, choice of substrates and microbial analysis.

Keywords

Biohydrogen, soybean straw, Clostridium thiosulpha-tireducens, Enterobacter aerogenes

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