IJEP 45(6): 500-509 : Vol. 45 Issue. 6 (June 2025)
1. Avinashilingam Institute for Home Science and Higher Education for Women, Department of Biochemistry, Biotechnology and Bioinformatics, Coimbatore – 641 108, Tamil Nadu, India
2. PSG College of Arts and Science, Department of Biotechnology, Coimbatore – 641 014, Tamil Nadu, India
Abstract
The residual analysis of insecticides in Luffa acutangula, commonly known as sponge gourd, is essential for ensuring food safety and evaluating potential health risks associated with pesticide exposure. This analysis is especially crucial considering the growing number of agricultural practices that significantly rely on chemical pest control. Utilizing the QuEChERS (quick, easy, cheap, effective, rugged and safe) method, samples of Luffa acutangula were prepared for analysis, allowing for the efficient extraction and cleanup of pesticide residues. Following sample preparation, liquid chromatography-mass spectrometry (LC-MS) was used for a comprehensive analysis. This technique is highly accurate and sensitive, thus making it an ideal choice for detecting trace amounts of residual pesticides in complex biological matrices. To further understand the implications of these residues on human health, molecular docking studies were conducted. These studies focused on assessing the interactions between the detected insecticides and key neurological enzymes. By examining these interactions, this approach not only elucidates the potential neurotoxic effects of these compounds but also contributes to the broader understanding of how agricultural practices may impact human health through food consumption. The findings from this study are crucial for informing regulatory policies that aim to maintain food safety and promote safer farming practices, thereby protecting consumers.
Keywords
Luffa acutangular, Liquid chromatography-mass spectrometry, Molecular docking, Neurological enzymes
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