A Pilot Scale Study on Immobilization of Chlorella sp. and Scenedesmus ecornis (Ehrenberg) Chodat on Banana Stem Matrix for Bioremediation of Chemical Industrial Effluent

IJEP 45(7): 639-645 : Vol. 45 Issue. 7 (July 2025)

Abishikha S. and S. Nandhini*

Ethiraj College for Women, Department of Microbiology, Chennai – 600 008, Tamil Nadu, India

Abstract

Effluent discharge from industries without treatment is one of the leading concerns for the environment, as it pollutes and has a negative impact on people’s quality of life, representing a global environmental problem. This study is aimed at exploring the bioremediation potential of microalgae Chlorella sp. and Scenedesmus ecornis (Ehrenberg) Chodat immobilized on a natural carrier, banana stem matrix, in order to improve the quality of industrial effluent and eliminate harmful metabolites. Industrial effluent sample was collected from chemical industry and analyzed for various physico-chemical parameters, such as pH, total dissolved solids (TDS), total suspended solids (TSS), biological oxygen demand (BOD), chemical oxygen demand (COD), total nitrogen, sulphate and phosphorus, pre- and post-treatment. For the industrial effluent treated using suspended Chlorella sp. culture, the treatment efficiency after 192 hr was found to be 87.5% of TSS, 94.9% of BOD, 93.2% of COD and 77.5% of total phosphorus. The treatment efficiency of immobilized Scenedesmus ecornis (Ehrenberg) Chodat after 24 hr was 28.9% of TSS, 16.99% of COD, 3.98% of sulphate, 98.7% of total phosphorus and 20.6% of total nitrogen. The treatment efficacy of both Chlorella sp. and Scenedesmus ecornis (Ehrenberg) Chodat was found to be statistically significant when compared to the control. This work could be useful in the development of cost-effective and environmentally friendly wastewater treatment methods.

Keywords

Wastewater, Microalgae, Immobilization, Bioremediation

References

  1. Zhou, L., et al. 2008. Recent patents on immobilized micro-organism technology and its engineering application in wastewater treatment. Rec. Pat. Eng., 2: 28- 35.
  2. Bashan, L.E. and Y. Bashan. 2010. Immobilized microalgae for removing pollutants: review of practical aspects. Bioresour. Tech., 101: 1611–1627.
  3. Ahmed, N.E., S.S. Salem and A.H. Hashem. 2022. Statistical optimization, partial purifcation and characterization of phytase produced from Talaromyces purpureogenus NSA20 using potato peel waste and its application in dyes decolourization. Biointerface Res. Appl. Chem., 12: 4417–4431.
  4. Fouda, A., et al. 2015. Biodegradation and detoxification of bisphenol-A by flamentous fungi screened from nature. J. Adv. Biol. Biotech., 2: 123–132.
  5. Hashem, A.H., E. Saied and M.S. Hasanin. 2020. Green and ecofriendly bio-removal of Methylene Blue dye from aqueous solution using biologically activated banana peel waste. Sustain. Chem. Pharm., 18:100333. DOI: 10.1016/j.scp.2020.100333.
  6. El-Sheekh, M.M., H.Y. El-Kassas and S.S. Ali. 2025. Microalgae-based bioremediation of refractory pollutants: An approach towards environmental susta-inability. Microbial Cell Factories. 24(1): 19. DOI: 10.1186/s12934-024-02638-0.
  7. Godjevargova, T., S. Mihova and K. Gabrovska. 2004. Fixed-bed biosorption of Cu2+by polyacrylonitrile-immobilized dead cells of Saccharomyces cerevisiae. World J. Microbiol. Biotech., 20: 273–279.
  8. El-Sheekh, M.M., et al. 2017. Effect of algal cell immobilization technique on sequencing batch reac-tors for sewage wastewater treatment. Int. J. Env. Res., 11: 603–611.
  9. El-Sheekh, M.M., et al. 2019a. Green technology applications for algal bloom control. In Handbook of algal technologies and phytochemicals. volume II: phycoremediation, biofuels and global biomass production. Ed G.A. Ravishankar, R.R. Ambati. Taylor and Francis, CRC Press, London. pp 13–2237.
  10. Kumar, A., et al. 2011. Review on bioremediation of polluted environment: A management tool. Int. J. Env. Sci., 1: 1079-1093.
  11. Mertens, B. and N.V. Boon. 2006. Slow-release inoculation allows sustained biodegradation of c-hexa-chlorocyclohexane. Appl. Env. Microbiol., 72: 622–627.
  12. Ma, C., et al. 2016. Removal of environmental estrogens by bacterial cell immobilization technique. Chemosphere. 144: 607–614.
  13. Mulla, S.I., et al. 2013. Enhanced degradation of 2-nitrotoluene by immobilized cells of Micrococcus sp. strain SMN-1. Chemosphere. 90: 1920–1924.
  14. Akhtar, N., J. Iqbal and M. Iqbal. 2004b. Enhancement of lead (II) biosorption by microalgal biomass immobilized onto loofa (Luffa cylindrica) sponge. Eng. Life Sci., 4: 171–178.
  15. El-Sheekh, M.M., et al. 2020. Simulation treatment of industrial wastewater using microbiological cell immobilization technique. Iran J. Sci. Tech. Trans. Sci., 44: 595–604. DOI: 10.1007/s40995-020-0 0866-8.
  16. Akbar, N. A., et al. 2019. Removal of colour using banana stem adsorbent in textile wastewater. J. Physics: Conf. Series. 1349. DOI: 10.1088/1742-6596/1349/1/012091.
  17. Baskaran, V. and M. Nemati. 2006. Anaerobic reduction of sulphate in immobilized cell bioreactors, using a microbial culture originated from an oil reservoir. Biochem. Eng. J., 31:148–159.
  18. Wang, Y., et al. 2007. Nutrition and biodegradation of phenol by free and immobilized Acinetobacter sp. strain PD12. J. Env. Sci., 19: 222–22.
  19. Mkpuma, V.O., N.R. Moheimani and H. Ennaceri. 2024. Biofilm and suspension-based cultivation of microalgae to treat anaerobic digestate food effluent (ADFE). Sci. Total Env., 171320.
  20. Rajasulochana, P., et al. 2009. Bioremediation of oil refinery effluent by using Scenedesmus obliquus. J. American Sci., 5: 17-22. 
  21. Mittal, S. and R.M.S. Sengar. 1989. Toxic effect of sulphate and its uptake in algae. Natl. Acad. Sci. Lett., 12: 17-19.