IJEP 45(7): 646-651 : Vol. 45 Issue. 7 (July 2025)
Dr M.G.R. Educational and Research Institute, Department of Biotechnology, Chennai – 600 095, Tamil Nadu, India
Abstract
This research focuses on experimenting with isolated microbes from oil-contaminated environments and evaluating their ability to analyze crude oil biodegradation activity on a laboratory scale using different medium sources. The indigenous microbes were cultured in a selective medium using crude oil and were identified using 16S rRNA sequencing obtained with Bacillus subtilis subsp. stercoris and Enterobacter ludwigii strains. Furthermore, the growth of bacterial isolates was observed in seawater. Seawater contained Luria-Bertani (LB) medium and Bushnell Haas (BH) medium. A combination of LB and BH medium was cultured with crude oil for 7 days at 37°C in a rotary shaker (120 rpm) and assessed for growth kinetics to evaluate their stability and degradation of crude oil. The results revealed that the microbes in the LB-BH medium had better nutrient availability and oil-degrading capabilities, which led to the next steps in the deterioration process. GCMS studies were used to evaluate the degraded crude oil residues. The data revealed that the microbes utilized the provided medium sources and 51.89% degradation of crude oil was detected in the experiment.
Keywords
Seawater, Crude oil, Luria-Bertani, Bushnell Haas, Consortium, Biodegradation
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