Biodegradation Of Diesel By Pseudomonas stutzeri DBT15 Strain Isolated From Contaminated Soil Of Mathura

IJEP 41(2): 179-186 : Vol. 41 Issue. 2 (February 2021)

Pooja Pandey1, Hardik Pathak1, Saurabh Dave2* and D.P. Jaroli3

1. JECRC University, Department of Biotechnology, School of Science, Jaipur – 303 905, Rajasthan, India
2. JECRC University, Department of Chemistry, School of Science, Jaipur – 303 905, Rajasthan, India
3. Board of Secondary Education, Ajmer, Rajasthan, India


A bacterial strain was isolated from petroleum contaminated soil of Mathura. Total 5 soil samples were collected and 20 diesel-oil degrading bacteria were isolated. On the basis of different biodegradation capabilities of different bacterial species, Pseudomonas stutzeri DBT15 was selected using 2,6 dichlorophenol indophenol (DCPIP) assays. The biodegradation potential was evaluate by gravimetric method and residual oil was analyzed using gas chromatography/mass spectrometry (GC/MS) analysis. 96.18% of degradation was observed for M15 bacterial strain in 35 days of incubation by gravimetric analysis. Molecular characterization was performed by 16S rRNA nucleotide sequencing and it was identified as Pseudomonas stutzeri DBT15 strain.


GC-MS, Pseudomonas stutzeri, 2,6 dichlorophenol indophenols


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