IJEP 41(2): 179-186 : Vol. 41 Issue. 2 (February 2021)
Pooja Pandey1, Hardik Pathak1, Saurabh Dave2* and D.P. Jaroli3
1. JECRC University, Department of Biotechnology, School of Science, Jaipur – 303 905, Rajasthan, India
2. JECRC University, Department of Chemistry, School of Science, Jaipur – 303 905, Rajasthan, India
3. Board of Secondary Education, Ajmer, Rajasthan, India
Abstract
A bacterial strain was isolated from petroleum contaminated soil of Mathura. Total 5 soil samples were collected and 20 diesel-oil degrading bacteria were isolated. On the basis of different biodegradation capabilities of different bacterial species, Pseudomonas stutzeri DBT15 was selected using 2,6 dichlorophenol indophenol (DCPIP) assays. The biodegradation potential was evaluate by gravimetric method and residual oil was analyzed using gas chromatography/mass spectrometry (GC/MS) analysis. 96.18% of degradation was observed for M15 bacterial strain in 35 days of incubation by gravimetric analysis. Molecular characterization was performed by 16S rRNA nucleotide sequencing and it was identified as Pseudomonas stutzeri DBT15 strain.
Keywords
GC-MS, Pseudomonas stutzeri, 2,6 dichlorophenol indophenols
References
- Pandey, P., H. Pathak and S. Dave. 2016. Micro bial ecology of hydrocarbon degradation in the soil: A review. Res. J. Env. Toxicol., 10:1-15.
- Collins, C. 2007. Implementing phytoremediation of petroleum hydrocarbons. In Methods in biotech. Humana Press, New York.
- Lee, M., et al. 2006. Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurens is EN3 in the presence of mycolic acid. J. Appl. Microbiol., 1364-5072.
- Mariano, A.P., et al. 2007. Laboratory study on the bioremediation of diesel oil contaminated soil from a petrol station. Brazilian J. Microbiol., 38:346-353.
- Gallego, J.L., et al. 2001. Bioremediation of diesel-contaminated soils: Evaluation of potential in situ techniques by study of bacterial degradation. Biodegradation. 12:325-335.
- Lawson, I.Y.D., E.N. Atibila and S.K.A. Danso. 2015. Enhancement of diesel oil degradation by nitrogen and phosphorus fertilizers in four ghanaian soils. J. Ghana Sci. Assoc.,16: 52-68.
- Baheri, H. and P. Meysami. 2002. Feasibility of fungi bioaugmentation in composting a flare pit soil. J. Hazard. Mater., 89:279–286.
- Varjani, S.J., and V.N. Upasani. 2013. Comparative studies on bacterial consortia for hydrocarbon degradation. Int. J. Innovative Res. Sci., 10: 5377-5383.
- Magdalena, P.P., G.A. Plaza and A. Poliwoda. 2014. Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil. Env. Sci. Poll. Res., 21:9385-9395.
- Cao, J., Q. Lai and J. Yuan. 2015. Genomic and metabolic analysis of fluoranthene degradation pathway in Celeribacter indicus P73T. Sci. Rep., 13:7741-7753.
- Chaillan, F., A. Le Fleche and E. Bury. 2004. Identification and biodegradation potential of tropical aerobic hydrocarbon degrading microorganisms. Res. Microbiol., 7:587-595.
- Daugulis, A.J. and C.M. McCracken. 2003. Microbial degradation of high and low molecular weight polyaromatic hydrocarbons in a two-phase partitioning bioreactor by two strains of Sphingomonas sp. Biotech. Letters. 17:1441-1444.
- Bharathi, S. and N. Vasudevan. 2001. Utilization of petroleum hydrocarbons by Pseudomonas fluorescens isolated from petroleum contaminated soil. Env. Int., 26.
- Cunha, C.D. and S.G.F. Leite. 2000. Gasoline biodegradation in different soil microcosms. Brazilian J. Microbiol., 31:45-49.
- Attiogbe, F.K., M.G. Amengor and K.T. Nyadziehe. 2007. Correlating biochemical and chemical oxygen demand of effluents : a case study of selected industries in Kumasi, Ghana, West Africa. J. Appl. Ecol., 11:110-118.
- Bidoia, E.D., R.N. Montagnolli and P.R.M. Lopes. 2010. Microbial biodegradation potential of hydrocarbons evaluated by colorimetric technique: a case study. Corpus ID:43538983.
- Mooti, A.E.L., et al. 2005. Isolation and identification of bacterial consortia responsible for degrading oil spills from the coastal area of Yanbu, Saudi Arabia. Biotech. Biotech. Equipment. 30(1):69-74.
- Yoshida, N., et al. 2001. Improvement of a mediator-type biochemical oxygen demand sensor for on-site measurement. J. Biotech., 88:269-275.
- Udgire, M., N. Shah and M. Jadhav. 2015. Enrichment, isolation and identification of hydrocarbon degrading bacteria. Int. J. Current Microbiol. Appl. Sci., 4:708-713.
- Bushnell, L.D. and H.F. Haas. 1994. The utilization of certain hydrocarbons by microorganisms. J. Bacteriol., 41: 653-673.
- Youssef, M., et al. 2010. Hydrocarbon degrading bacteria as indicator of petroleum pollution in Ismailia canal, Egypt. World Appl. Sci. J., 8:1226-1233.
- Zhang, R., et al. 2005. Diversity of organophosphorus pesticide degrading bacteria in polluted soil and conservation of their organophosphorus hydrolase genes. Canadian J. Microbiol., 51(4):337-343.
- Saxena, M.M. 1990. Environmental analysis: Water, soil and air. Agro Botanical Publishers.
- Zanaroli, G., et al. 2010.Characterization of two diesel fuel degrading microbial consortia enriched from a non-acclimated, complex source of microorganisms. Microbial Cell Fact., 9:1-13.
- Mariano, A.P., D.M. Bonotto and D.F. De-Angelis. 2008. Biodegradability of commercial and weathered diesel oils. Brazilian J. Microbiol., 39:133-142.
- Afifi, A., H. Motamedi and H. Alizadeh. 2015. Isolation and identification of oil degrading bacteria from oil sludge in Abadan oil refinery. Env. Experimental Biol., 13:13-18.