Production Of Biopreservative Using Lawsonia inermis And Evaluating Their Toxicity

IJEP 41(3): 250-256 : Vol. 41 Issue. 3 (March 2021)

Jeba Sweetly Dharmadhas*, Sumayya Rehaman, Issac Abraham Sybiya Vasantha Packiavathy, Jeyapragash Danaraj and Archana Ganesan

Karpagam Academy of Higher Education, Department of Biotechnology, Coimbatore – 641 021, India

Abstract

Salt preservation is the general and old age popular practice. Alternate methods of preservations have been analyzed in order to reduce the pollution load in the soaking process of leather by many researchers. Due to the stringent pollution of total dissolved solids (TDS) and salinity in tannery effluents, there is a need to develop and adopt salt-free curing methods suitable for Indian raw material. Chilling is the most commonly employed short term preservation method in many countries and is considered more efficient and cost-effective than biocides based methods. In the current study, plant derived extract from Lawsonia inermis was used as salt-less formulations for curing goat skins, proving an effective alternative to the conventional preservation with NaCl. The physico-chemical properties of all goat skin were tested and the results met the standard requirements. After treatment with plant extract, the pollution loads of soaking liquors were assessed and there was a dramatic reduction in levels of total solids, total suspended solids (TSS), chloride, total dissolved solids (TDS), biological oxygen demand (BOD) and chemical oxygen demand (COD) released. From the results, it is clear that the proposed preservation technique is completely comparable with the traditional salt curing approaches, which may be used as viable alternative options for skin preservation in leather processing industries.

Keywords

Sodium chloride, Lawsonia inermis, Goat skin, Antimicrobial, Leather processing

References

  1. Sarker, K. T. 1995. The theory and practice of leather manufacture (2nd edn). Longman Publishers, India.
  2. Sivabalan, V. and A. Jayanthi. 2009. A study to reduce salt usage in preservation of skins and hides with alternate use of plant extract. J. Agric. Biol. Sci., 4(6): 43-48.
  3. Slabbert, N. P. 1979. Recycling in the tanning industry. J. Soc. Leather Tech. Chem., 64: 89-92.
  4. Franco, J., et al. 2019. A review of eco-friendly preservative and biotannin materials using powdered barks of local plants for the processing of goat skin. Int. Res. J. Sci. Tech., 1: 13-20.
  5. Covington, A. 2009. Tanning chemistry: The science of leather. Royal Society of Chemistry, Cambridge. pp 72.
  6. Kanagaraj, J., et al. 2001. Cleaner techniques for the preservation of raw goat skins. J. Cleaner Production. 9: 261-268.
  7. Daniels, R. 1997. Overview: Avoiding salts. World Leather. 10: 41-45.
  8. Kanagaraj, J. and B. Chandra. 2002. Alternative to salt curing techniques – A review. J. Sci. Industrial Res., 61: 339-348.
  9. Babu, N. K. C., et al. 2012. A systematic study on the role of chilling temperatures on the curing efficacy of hides and skins. J. American Leather Chem. Assoc., 107(11): 362-370.
  10. Gudro, I., et al. 2014. Short-term preservation of hide using vacuum. Plos. One. 9(11): 1-9.
  11. Sathish, M., et al. 2013. Dry ice – An eco-friendly alternative for ammonium reduction in leather manufacturing. J. Clean. Prod., 54: 289-295.
  12. Venkatachalam, P., et al. 1982. Further experiments in salt-less curing. Leather Sci., 29: 217-221.
  13. Aloy, M. 1998. Cleaner tanning technologies in the beam house operation. Symposium on Cleaner tanning technology. Proceedings, pp 6.
  14. Shegaw, M. A., et al. 2016. Rumex abyssinicus (mekmeko) Ethiopian plant material for preservation of goat skins: Approach for cleaner leather manufacture. J. Clean. Prod., 133: 1043-1052.
  15. Vedaraman, N., et al. 2016. De-oiled neem cake as potential bio-additive for low salt raw skin preservation: A process for salinity reduction in tanneries. Int. J. Env. Sci. Tech., 13: 1563-1572.
  16. Madhan, A., et al. 2018. Leaf paste aided goat skin preservation: Significant chloride reduction in tannery. J. Env. Chem. Eng., 6: 4423-4428.
  17. Madhan, A., et al. 2017. Sodium chloride substitute for lower salt goat skin preservation: A novel approach. J. American Leather Chem. Assoc., 112: 270-276.
  18. Vijayalakshmi, K., et al. 2009. Novel plant based formulations for short term preservation of animal skins. J. Sci. Ind. Res., 68: 699-707.
  19. Hashem, M. A., et al. 2018. Leaf paste aided goat skin preservation: Significant chloride reduction in tannery. J. Env. Chem. Eng., 6(4): 4423-4428.
  20. Shede, P. N., et al. 2008. Bacterial succession on raw buffalo hide and their degradative activities during ambient storage. Int. Biodeterioration Biodegradation. 62(1): 65-74.
  21. BIS. 2007. Methods of test for vegetable tanning materials. Bureau of Indian Standards, New Delhi.
  22. Ilze, G., et al. 2015. Raw hide preservation using vacuum under low temperature. Ph.D. Thesis. Riga Technical University.
  23. APHA. 2012. Standard methods for the examination of water and wastewater. American Public Health Association, Washington, D.C.
  24. Drury, R. A. B. and E. A. Wallington. 1980. Carleton’s histology technique (5th edn). Oxford University Press, New York, Toronto.
  25. Manual of veterinary investigation laboratory techniques. Part 5: Histology. 1981. Ministry of Agriculture, Fisheries and Food. U. K. Reference Book. pp 366.
  26. Pekhtasheva, E., et al. 2012. Biodamages and protection of leather and fur. Chem. Chem. Tech., 6: 3.
  27. Birbir, Y., et al. 2008. Direct electric current utilization in destruction of extremely halophilic bacteria in salt that is used in brine curing of hides. J. Electrostatics. 66: 388-394.