IJEP 41(5): 495-502 : Vol. 41 Issue. 5 (May 2021)
Shalini and Y. Pydi Setty*
National Institute of Technology, Department of Chemical Engineering, Warangal – 506 004, Telangana, India
A large amount of congo red (CR) has been discharged into the environment, mostly from the textile industries. The current study aims to find out the potential approach of congo red (CR) dye decolourization using suspended and immobilized B. subtilis. The effect of parameters (dye concentration, pH and temperature) on dye decolourization using suspended cells was studied. The corresponding cell mass (OD600) alongwith the decolourization profile, was considered to understand the effect of cell mass. B. subtilis immobilized on polyurethane foam (PUF) cubes (size: 1 cm3) have chosen to investigate the decolourization efficiency. SEM results revealed the porous structure of PUF and layer formation. The FTIR analysis was employed to confirm the decolourization. The maximum decolourization of 92% was achieved by immobilization method within 6 hr, whereas suspended cell assisted decolourization showed 82% within 12 hr. The data confirmed the second-order decolourization kinetics. We have found that the reaction rate and reaction rate constant (k) was found to be higher for immobilized cell assisted decolourization. The characteristic azo peaks have not found in FTIR samples of immobilized decolourization. The results confirmed that immobilization of B. subtilis is an efficient method for CR decolourization compared to the suspended cells.
Bacillus subtilis, Congo red, Immobilization on polyurethane foam, microbial assisted decolourization
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