IJEP 42(1): 65-70 : Vol. 42 Issue. 1 (January 2022)
N. Ramya, Sathish Shankar Bhat and P.K. Maheshwar*
University of Mysore, Yuvaraja’s College, Department of Microbiology, Mysore – 570 005, Karnataka, India
Farmers handling pesticides without following the instructions or spraying them without any personal protective equipment are more prone to pesticide exposure. Monocrotophos is one of the extensively used as well as highly hazardous organophosphorus insecticides. It is known to inhibit the acetylcholinesterase enzyme, which plays an important role in the hydrolysis of the neurotransmitter acetylcholine at the cholinergic synapses. This research aims to calculate the cholinesterase level as a biomarker to compare the pesticide exposure level in the blood of farmers and a comparative variation in cholinesterase level before and after six months of pesticide exposure. The results indicated a considerable decrease in the cholinesterase levels in the blood serum of farmers after exposure to monocrotophos insecticide as compared to the non-exposure time. Percentage depression of cholinesterase is found to be high in young spray farmers. Statistical evaluation results (t=33.231) also confirmed depression in the cholinesterase level is highly significant. The p-value for the decrease in cholinesterase was found to be less than α=0.05, that is 0.009<0.05.
Monocrotophos, Blood sampling, Acetylcholinesterase, Cholinesterase, Pesticide
- Wilson, C. 1999. Cost and policy implications of agricultural pollution with special reference to pesticides. Ph.D. Thesis. Department of Economics, University of St. Andrews, Scotland, UK.
- Vega, S.S. 1994. Note on the toxicity of pesticides used in tropical crops. Ciencias Ambientales. 11: 181.
- Moreno-Banda, et al. 2009. Maternal exposure to floricultural work during pregnancy, PON1 Q192R polymorphisms and the risk of low birth weight. Sci. Total Env., 407(21): 5478-5485.
- Pilkington, A., et al. 2001. An epidemiological study of the relations between exposure to organophosphate pesticides and indices of chronic peripheral neuropathy and neuropsychological abnormalities in sheep farmers and dippers. Occup. Env. Med., 58(11): 702-710.
- Rama, D.B.K. 1995. Cholinesterase enzyme reaction and inhibition by pesticides. African Newsletter Occup. Health Saf., 5: 17-19.
- Roldan-Tapia, L. 2006. Neurophysiologial sequela from acute poisoning and long term exposure to carbamate and organophosphate pesticides. Neurotoxicol. Teratol., 28: 694-703.
- Pathak, M.K., et al. 2011. Cholinesterase levels and morbidity in pesticide sprayers in North India. Occup. Med., 61(7): 512-514.
- Rama, D.B. and K. Jaga. 1992. Pesticide exposure and cholinesterase levels among farmworkers in the Republic of South Africa. Sci. Total Env., 122: 315-319.
- Fareed, M., et al. 2013. Adverse respiratory health and hematological alterations among agricultural workers occupationally exposed to organophosphate pesticides: A cross-sectional study in North India. PLoS ONE. 8(7): e69755.
- Remor, A.P., et al. 2009. Occupational exposure of farmworkers to pesticides: Biochemical parameters and evaluation of genotoxicity. Env. Int., 35: 273-278.
- McConnell, R. and R. Magnotti. 1994. Screening for insecticide overexposure under field conditions-A re-evaluation of the tintometric cholinesterase kit. American J. Public Health., 84: 479-481.
- Catano, H.C., et al. 2008. Plasma cholinesterase levels and health symptoms in Peruvian farm workers exposed to organophosphate pesticides. Arch. Env. Contam. Toxicol., 55: 153-159.
- Slotkin, T.A. and F.J. Seidler. 2012. Developmental neurotoxicity of organophosphates targets cell cycle and apoptosis, revealed by transcriptional profiles in-vivo and in-vitro. Neurotoxicol. Teratol., 34(2): 232-241.
- Chambers, J.E., et al. 1992. Organophosphates, chemistry, fate and effects. Academic Press, San Diego, USA.
- Luster, M.I. and G.J. Rosenthal. 1993. Chemical agents and the immune response. Env. Health Perspect., 100:219-226.
- Vial, T., B. Nicolas and J. Descotes. 1996. Clinical immunotoxicity of pesticides. J. Toxicol. Env. Health. 48(3):215-229.
- Cocker, J., et al. 2002. Biological monitoring of exposure to organophosphate pesticides. Toxicol. Lett., 134(1-3):97-103.
- Kwong, T. 2002. Organophosphate pesticides: Biochemistry and clinical toxicology. Ther. Drug Monit., 24: 144-149.
- Melissa, K., et al. 2009. Standard operating procedures for serum and plasma collection: Early detection research network consensus statement standard operating procedure integration working group. J. Proteome Res., 8(1): 113-117.