IJEP 42(11): 1292-1298 : Vol. 42 Issue. 11 (November 2022)
1. Ethiraj College for Women, Department of Microbiology, Chennai, Tamil Nadu – 600 008, India
2. Sugam Hospital, Chennai, Tamil Nadu – 600 019, India
Pseudomonas fluorescens is a widely dispersed plant growth promoting rhizobacteria. It is not a human or plant pathogen and is found in soil, water, plants and other moist environments. This organism has already been used as a biocide against various fungi and bacteria in agricultural fields . It has lots of biocontrol properties, like antibiotics, enzymes and siderophore productions. Due to its siderophore production its pyoverdine pigment fluoresces under UV light. This study was conducted mainly to construct a media with the cheapest local resource or agricultural waste and also to find its application as a biopreservative. The raw materials required for the construction of medium were agricultural wastes, such as rice husk, rice straw, wheat husk, peanut waste, dried cow dung and then coconut water, whey water and vermicompost. Then these four combinations were inoculated from the mother inoculum and their growth was checked by serial dilution and spread plate technique. Then these cultures were applied to apples, tomatoes, curry leaves and betel leaves by dip inoculation method. The keeping qualities of these vegetables were checked till the control items were rotten under laboratory conditions approximately for about 7 days. The physiological characteristics were good and fresh in the preserved items for 7 days but decayed on the control products. Thus the first step towards the application of P. fluorescens as a biopreservative was successful.
Pseudomonas fluorescens, Agricultural wastes, biopreservative, Fruits, Vegetables, environment
- Reddy, B.P. and M.S. Reddy. 2009. Isolation of secondary metabolities from Pseudomonas fluorescens and its characterization. Asian J. Res. Chem., 2(1):26-29.
- Vijayalakshmi, K. 2016. Studies on degradation of a tannery textile dye erichrome blue black by Pseudomonas fluorescens. Res. J. Pharm. Tech., 9(4):445-450.
- Angayarkanni, T., et al. 2012. Antimicrobial compounds production by Pseudomonas fluorescens and Bacillus subtilis. Asian J. Res. Chem., 5(1):61-64.
- Ahmed, E.G., et al. 2002. Biological control of postharvest diseases of fruits and vegetables. J. Mycol. Biotech., 2:219-238.
- Bhaskar, G. and Y. T. Gaikwad. 2015. Microbial treatment to black liquor. Asian J. Res. Chem., 8(2):113-116. DOI:5958/0974-4150. 2015.000 26.6.
- Subathra Devi, C., et al. 2014. Optimization studies on nattokinase enzyme production by a mutant strain Pseudomonas aeruginosa CMSS isolated from bovine milk. Res. J. Pharm. Tech., 7(11): 1297-1299.
- Chang, C.M., M.P. Doyle and J.B. Lunchansky. 1995. Identification of Pseudomonas fluorescens strains isolated from raw pork and chicken that produce siderophores antagonistic towards food borne pathogens. J. Food Prot., 58(12):1340-1344.
- Kandan, A., et al. 2002. Induction of phenyl propanoid metabolism by Pseudomonas fluorescens against tomato spotted wilt virus in tomato. Folia Microbiol., 47(2):121-129.
- Jayaraman, J., T. Parthasarathi and N.V. Radha krishnan. 2006. Characterization of a Pseudomonas fluorescence strain from tomato rhizosphere and its use for integrated management of tomato damping-off. J. Crop Prot., 52(5):683-702.
- Saikia, N., et al. 2017. Microbial and biochemical analysis of fresh fruits, vegetables and packaged drinks. Res. J. Pharm. Tech., 10(9):2952-2954. DOI:10.5958/0974-360X.2017.00523.6.
- Venkateswarulu, T.C., et al. 2015. Bioethanol production by yeast fermentation using pomace waste. Res. J. Pharm. Tech., 8(7):841-844. DOI: 10.5958/0974-360X.2015.00137.7.
- Vivekananthan, R., et al. 2004. Lytic enzymes induced by Pseudomonas fluorescens and other biocontrol organisms mediate defense against the anthracnose pathogen in mango. World J. Microbiol. Biotech., 20:235-244.
- Vipin, C., et al. 2017. Overproduction of pyocyanin in Pseudomonas aeruginosa by supplementation of pathway precursor shikimic acid and evaluation of its activity. Res. J. Pharm. Tech., 10(2): 533-536.
- Arunkumar, T., N. kumar and A. Anand. 2017. Statistical evaluation of medium components by Plackett-Burman method for laccase from Pseudo-monas aeruginosa ADNO4 using submerged fermentation. Res. J. Pharm. Tech., 10(4):1115-1119.
- Subbaiya, R., et al. 2018. Application of response surface methodology in process parameter optimization of media for production of amylase. Res. J. Pharm. Tech., 11(12):5273-5281.
- Poorni, K.E., et al. 2011. Production of Pseudo-monus fluorescens from agricultural wastes and its application in the preservation of selected vegetables. Adv. Appl. Sci., 2(2):156-160.
- Rachida, Z. A., et al. 2017. Screening of phenolic compounds from Abelmoschus esculentus L. extract fruits and in-vitro evaluation of antioxidant and antibacterial activities. Res. J. Pharm. Tech., 10(12):4371-4376. DOI:10.5958/0974-360X. 2017-00804.6.
- Sandeep, D.S., et al. 2016. Formulations of antimicrobial polyherbol handwash. Res. J. Pharm. Tech., 9(7):864-866. DOI:10.5958/0974-360x. 2016.00.163.3.
- Olanya, O.M., et al. 2013. Efficacy of Pseudomonas fluorescens for biocontrol of E. coli 0157:H7 on spinach. J. Food Agric. Env., 11(2):86-91.