IJEP 42(6): 745-751 : Vol. 42 Issue. 6 (June 2022)
1. Dr. M.G.R. Educational and Research Institute, Department of Biotechnology, Maduravoyal, Chennai – 600 095, Tamil Nadu, India
2. Dr. M.G.R. Educational and Research Institute, Department of Civil Engineering, Maduravoyal, Chennai – 600 095, Tamil Nadu, India
Abstract
This paper enumerates the production of lignolytic extracellular enzyme, laccase enzyme from the fungal species- Pleurotus sajorcaju (an edible lignolytic mushroom) and usage of its agro residues as a potent biofertilizer. Fungus have been obtained and subcultured in potato dextrose agar and enzyme activity is checked using a substrate, guaiacol (phenolic compound). Changes in the Petri dishes, zonal formation around the mycelia discs shows the oxidizing property of the enzyme after the guaiacol addition is checked for a week and graph is plotted showing the differences. Solid state fermentation is carried on using various substrates (rice bran, wheat bran, wood powder, coconut coir, green pea husk, paper, sugarcane bagasse). Enzyme activity assay is done for all the above content assayed by guaiacol and protein activity assay (Bradford protein assay) is done, prognosis showed that the amount of enzyme produced by P. sajorcaju is high in green pea husk infused fermentation media. Temperature and pH optimization has been done and the results are compared between the test and control. Results are plotted in graph in accordance with optical density (OD), temperature and pH, respectively. Purification and characterization of laccase enzyme is done implementing the methods dialysis and SDS-PAGE, respectively. The agro residual waste, leftover residue after the extraction of media is screened for C,P,N,K content to ensure its biofertilizing capacity.
Keywords
Laccase, Pleurotus sajorcaju, Agro residues, Lignocellulosic substrates
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