Potential Laccase Producer (Pleurotus Sajorcaju)

IJEP 42(6): 745-751 : Vol. 42 Issue. 6 (June 2022)

B. Priyadharshini1, R. Vishali1, A. Anitha1, G.R. Sujithra1, Kowsalya1*, K.G. Purushotham1 and R.M. Narayanan2

1. Dr. M.G.R. Educational and Research Institute, Department of Biotechnology, Maduravoyal, Chennai – 600 095, Tamil Nadu, India
2. Dr. M.G.R. Educational and Research Institute, Department of Civil Engineering, Maduravoyal, Chennai – 600 095, Tamil Nadu, India


This paper enumerates the production of lignolytic extracellular enzyme, laccase enzyme from the fungal species- Pleurotus sajorcaju (an edible lignolytic mushroom) and usage of its agro residues as a potent biofertilizer. Fungus have been obtained and subcultured in potato dextrose agar and enzyme activity is checked using a substrate, guaiacol (phenolic compound). Changes in the Petri dishes, zonal formation around the mycelia discs shows the oxidizing property of the enzyme after the guaiacol addition is checked for a week and graph is plotted showing the differences. Solid state fermentation is carried on using various substrates (rice bran, wheat bran, wood powder, coconut coir, green pea husk, paper, sugarcane bagasse). Enzyme activity assay is done for all the above content assayed by guaiacol and protein activity assay (Bradford protein assay) is done, prognosis showed that the amount of enzyme produced by P. sajorcaju is high in green pea husk infused fermentation media. Temperature and pH optimization has been done and the results are compared between the test and control. Results are plotted in graph in accordance with optical density (OD), temperature and pH, respectively. Purification and characterization of laccase enzyme is done implementing the methods dialysis and SDS-PAGE, respectively. The agro residual waste, leftover residue after the extraction of media is screened for C,P,N,K content to ensure its biofertilizing capacity.


Laccase, Pleurotus sajorcaju, Agro residues, Lignocellulosic substrates


  1. Walker, J.W.R. and R.F. McCallion. 1980. The selectice inhibition of ortho and para phenol oxidases. Phytochem., 19:373.
  2. Cohen, R., L. Persky and Y. Hadar. 2001. Biotechnological application and potential of wood-degrading mushroom of the genus pleurotus. Appl. Microbiol. Biotech., 58:582-594.
  3. Sarkanen, K.V. and C.H. Ludwig. 1971. Lignins: Occurence, formation, structure and reaction. John Wiley and Sons Inc., New York.
  4. Kainz, S., et al. 2007. International patent W02003053375A.
  5. Paul, S. 2000. Growth parameters for gourmet and medicinal mushroom species (chapter 21). In Growth gourmet and medicinal mushrooms. Ten Speed Press, Berekely, California.
  6. Sigoillot, J.C., et al. 2012. Fungal strategies for lignin degradation. Adv. Botanical Res., 61:263-308.
  7. Khan, M., et al. 2016. Extracellular laccase from Pleurotu sajorcaju: Fermentative conditions and influence of nitrogenous sources. Indian J. Biotech., 15(2):230-235.
  8. Wongsangprai, C. 2012. Bag cultivation of mushroom (chapter 1). Kasetsiam, Bangkok. pp 7-9.
  9. Patel, H., A. Gupta and S. Gupte. 2009. Effect of different culture conditions and inducers on production of laccase by a basidiomycete fungal isolate Pleurotus ostreatus HP-1 under solid state fermentation. Bioresour., 4(1):268-284.
  10. Wolfenden, R.S. and R.L. Wilson. 1982. Radical- cations as reference chromogens in the kinetic studies of one-electron transfer reactions: Pulse radiolysis studies of 2,2-azinobis-(3-ethyl benzthia- zoline-6-sulphonate). J. Chem. Soc. Perkin Trans II. 2:805–812.
  11. Gupte, A. S. Gupte and H. Patel. 2007. Ligninolytic enzyme production under solid-state fermentation by white rot fungi. J. Sci. Ind. Res., 66:611-614.
  12. Eggert, C., et al. 1996. A fungal metabolite mediates degradation of non-phenolic lignin structures and synthetic lignin by laccase. FEBS Letters. 391:144-148.
  13. Xu, F., et al. 1999. Encyclopedia of bioprocess technology: Fermentation, biocatalysis, bioseparation. John Willey and Sons, Inc., New York. pp 1145-1154.
  14. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye bind-ing. Anal. Biochem.,72:248–254.
  15. Viswanath, B., et al. 2014. Fungal laccases and their applications in bioremediation. Enzyme
    DOI: 10.1155/2014/163242.
  16. Vasudev, K., et al. 2005. Biochemical characterization and molecular evidence of a laccase from the bird’s nest fungus Cyathus bulleri. Fungal Genetics Biol.,42(8):684-693.
  17. Patel, H. 2009. Effect of different culture conditions and induces on production of laccase by a basidiomycete fungal isolate Pleurotus ostreatus HP-1 under solid state fermentation. Bioresour., 268-284.
  18. IS 6092 (Part I). 1985. Methods of sampling and test for fertilizers (first revision). Indian Standard, New Delhi.