Bioremediation of Congo Red using wild and mutant Aspergillus flavus

IJEP 42(9): 1116-1121 : Vol. 42 Issue. 9 (September 2022)

Kavinkumar M.1*, Sivaselvam M.2, Dishonprabhu C.2, Iswariya Lakshmi P.2 and Lokesh P.1

1. Vellore Institute of Technology, School of Biosciences and Technology, Vellore – 632 014, Tamil Nadu, India
2. K.S. Rangasamy College of Arts and Science, Department of Biotechnology, Tiruchengode – 637 215, Tamil Nadu, India

Abstract

The main cause of aquatic pollution is the water effluents from the textile industries. To bioremediate the dye some micro-organisms can be used especially, the fungus present in the soil can bioremediate the dye. The fungal strains were isolated from the dye-affected soil. The soil sample was collected from the Noyal river near A. Valasu, taluk Kangayam, district Tirupur, Tamil Nadu, India. The isolated fungus was studied morphologically and confirmed as Aspergillus flavus then the fungus was subjected to physical and chemical mutagens, such as UV light and ethyl methyl sulphonate. Finally, the degradation capacity of the wild and mutant Aspergillus flavus was screened against congo red using calorimetry. it was observed that 70.58% of degradation was done by wild fungi whereas 78.43% of degradation was observed on mutant type with Congo red. Thus, it was proved that the mutant fungal strain could degrade the Congo red faster than wild-type strain.

Keywords

Fungi, mutation, Congo red, Bioremediation, Textile industry

References

  1. Dhanjal, N.I.K., et al. 2013. Biodegradation of textile dyes using fungal isolates. J. Env. Sci. Tech., 6(2):99-105.
  2. Rani, B., et al. 2014. Bioremediation of dyes by fungi isolated from contaminated dye effluent sites for bio-usability. Brazilian J. Microbial., 45(3):1055-1063.
  3. Lellis, B., et al. 2019. Effects of textile dyes on health and the environment and bioremediation potential of living organisms. Biotech. Res. Innovation. 3:275-290.
  4. Purohit, M.K. and P.V. Desai. 2014. Production, purification and in-silico characterization of azo reductase enzyme azor 1KF803342 from Pluraf-ibactor gergoviae involved in dye degradation. J. Bioremed. Biodeg., 5(2):1-7. DOI: 10.4172/2155-6199.1000217
  5. Ng, I.S., et al. 2013. Decolourization of textile azo dye and Congo Red by an isolated strain of the dissimilatory manganese-reducing bacterium Shewanella xiamenensis BCO1. Appl. Microbial. Biotech., 98(5). DOI: 10.1007/s 00253-013-5151-z.
  6. Kaushik, P. and A. Malik. 2008. Fungal dye decolourizations: Recent advances and nature potential. Env. Int., 35:127-141.
  7. Olaniyi, O.O., et al. 2015. UV mutagenesis of Aspergillus flavus for enhanced mannanase synthesis and catabolite decuction studies. Res. J. Microbial., 10(11):542-550. DOI:10.3923/jm.2015. 542550.
  8. Prabha, S., et al. 2016. Assessment of the impact of textile effluents on microbial diversity in Tirupur district, Tamil Nadu. Appl. Water Sci., 7:2267-2277.
  9. Zhao, L.H., et al. 2018. Increasing laccase activity of white rot fungi by mutagenesis and treating papermaking wastewater. IOP Conf. Series. Earth Env. Sci., 191:012053. DOI: 10.1088/1755-1315/191/1/012053.
  10. Kumar, A.K., et al. 2015. Use of combined UV and chemical mutagenesis treatment of Aspergillus terreus D 34 for hyper-production of cellulose degrading enzymes and enzymatic hydrolysis of mild-alkali pretreated rice straw. Bioresour. Bioprocess. 2:35. DOI: 10.1186/s040643-015-0062-8.
  11. Lark, D., et al. 2019. Enzymatic degradation and detoxification of azo dye Congo Red by a new laccase from Oudemansiella canarii. Bioresour. Tech., 289:121655.
  12. Ranjitha, J., et al. 2018. Detoxification of dyes by Aspergillus niger isolated from dye contaminated soil effluent from the sites of textile industry. Res. J. Chem. Env., 22(5):1-5.
  13. Asses, N., et al. 2018. Congo Red decolourization and detoxification by Aspergillus niger: Removal mechanisms and dye degradation pathway. Biomed. Res. Int., 2018(7):1-9. DOI: 10.1155/2018/3049 686.
  14. Arya, Arti. 2019. Fungal biodegradation: A study on process optimization for decolourization of industrial dyes. Res. J. Life Sci. Bioinf. Pharmaceutical Chem. Sci., 5(3):690-699. DOI: 10.26479/2019.0503.56.
  15. Kumar, V.V., et al. 2011. Screening and induction of laccase activity in fungal species and its application in dye decolorization. African J. Microbiol. Res., 5(11):1261-1267. DOI: 10.5897/AJMRI 0.894.
  16. Mohan, S.K., et al. 2015. Optimization studies on the biodegradation of textile dye Congo Red using fungal strains. Int. J. Res. Appl. Sci. Eng. Tech., 3: 169-172.
  17. Sharma, M.P., C.P. Kaushik and A. Kaushik. 2016. Screening and characterization of fungi isolated from textile effluent polluted soil samples for the decolorization of dye. J. Env. Res. Develop.,10(4): 717-722.
  18. Barragan, B.E., M.C. Costa and Carmen Marquez. 2006. Biodegradatin of azo dyes by bacteria inoculated on solid media: A environmental systems engineering. Dyes Pigments. 75:73-81.
  19. Upendra, R.S. and P. Khandelwal. 2016. Physical mutagenesis based strain improvement of Aspergillus sp. for ienhanced production of lovastatin. Int. J. Pharmacy Pharmaceutical Sci., 8(7):163-167.
  20. Chakraborty, S., et al. 2013. Decolorization and biodegradation of Congo Red dye by a novel white rot fungus Alternaria alternata CMERI F6. Biore-sour. Tech., 147:662-666.
  21. Singh, L. and V. p. singh. 2010. Biodegradation of textile dyes, Bromophenol Blue and Congo Red by fungus Aspergillus flavus. Env. Int. J. Sci. Tech., 5:235-242.
  22. Radha, S., et al. 2012. Development of mutant fungal strains of Aspergillus niger for enhanced production of acid protease in submerged and solid state fermentation. European J. Exp. Biol., 2(5): 1517-1528.
  23. Mohammed, H. A. 2015. Biodecolourization of Congo Red dye by using two species of fungi. Mesopotamia Env. J., 1(4):50-56.