In-Vivo Assessment To Behavioural, Morphological And Melanophoric Alteratations In Response To Exposure To Mercuric Chloride In Freshwater Fish, Channa punctatus (Bloch.)

IJEP 41(2): 229-235 : Vol. 41 Issue. 2 (February 2021)

Harmanpreet Kaur Gill* and Anish Dua

Guru Nanak Dev University, Aquatic Biology Laboratory, Department of Zoology, Amritsar, 143 005, Punjab, India


The present research focused on the acute and chronic toxicity of mercuric chloride to freshwater fish, Channa punctatus, understanding the behavioural, morphological changes and variations in melanophores. The static non-renewal acute toxicity assay was conducted by exposing the fishes to four test concentrations of mercuric chloride and 96 hr LC50 value alongwith 95% confidence limits were found to be 1.38 mg/L. The physico-chemical analysis of water was done using standard methods. The behavioural and morphological abnormalities were recorded with 8 hr observation per day during the period of 96 hr for control and exposed groups. The results showed the altered behaviour and morphological changes such as opaque eyes, erratic swimming, loosening of scales, mucous secretion and coloration changes was observed in exposed group of fishes. Three sub-lethal doses on the basis of LC50 value for chronic assay were selected (0.138 mg/L, 0.276 mg/L and 0.414 mg/L). The scales were harvested from control and exposed groups for melanophores studies by using compound microscope. The duration and concentration dependent significant variations (p £ 0.05) in the different forms of melanophores were observed and frequency of melanophores were calculated as mean ± S.E. The 5 different forms named punctate (P), punctostellate (PS), stellate (S), reticulostellate (RS) and Reticulate (R) were observed and there occurs decrease in dispersal forms of melanophores (reticulate and reticullostellate) and increase in aggregated forms of melanophores (punctate, punctostellate).


Mercuric chloride, Channa punctatus, Behaviour, Morphology, Melanophores, Punctate, Punctostellate, Stellate, Reticulostellate, Reticulate


  1. Pereira, P., et al. 2014. Fish eyes and brain as primary targets for mercury accumulation- A new insight on environmental risk assessment. Sci. Total Env., 494-495: 290-298. DOI: 10.1016/j.scitotenv. 2014.07.008.
  2. Macirella, R., et al. 2016. Effects of two sublethal concentrations of mercury chloride on the morphology and metallothionein activity in the liver of zebrafish (Danio rerio). Int. j. molecular sci., 17:1-16. DOI: 10.3390/ijms17030361.
  3. Amiri, M.H. and H. M. Shaheen. 2012. Chromatophores and colour revelation in the blue variant of the stamese fighting fish (Betta splenders). Micro., 43:159-169. DOI: 10.1016/j.micron.2011.07. 002.
  4. Biswas, S.P., A. G. Jadhao and N. V. Palande. 2014. Role of catecholamines and nitric oxide on pigment displacement of the chromatophores of freshwater snakehead teleost fish, Channa punctatus. Fish physiol. Biochem., 40: 457-467. DOI: 10.1007/s10695-013-9857-8.
  5. EPA. 1996. Fish acute toxicity test, freshwater and marine. Environmental Protection Agency, United States. pp 1-11.
  6. APHA/AWWA/WEF. 2005. Standard methods for the examination of water and wastewater (vol 21). American Public Health Association, American water works Association, Washington, U.S.A.
  7. EPA. 2002. Short-terms methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms (4th edn). Environmental Protection Agency, United States. pp 1-45.
  8. Maheshwari, S. 2017. Exploring new pollution indicators in fish body parts for biomonitoring and toxicological studies. Ph.D. Thesis, Guru Nanak Dev University, Amritsar.
  9. 9. Gupta, N. 2005. Mercuric chloride toxicity in a freshwater fish Channa punctatus (Bloch). Ph.D. Thesis, Guru Nanak Dev University, Amritsar, India.
  10. Kaur, R. and A. Dua. 2015. 96 hr LC50, behavioural alterations and histopathological effects due to wastewater toxicity in a fresahwater fish Channa punctatus. J. Env. Sci. poll. res., 22:5100-5110. DOI: 10.1007/s11356-014-3710-11.
  11. Rahimibashar, M.R. and V Alipoor. 2012. The determination of LC50 and bioconcentration of mercury chloride (HgCl2) in Esox lucius. World appl. sci. j., 17:735-738.
  12. Banavathu, S.N., N. N. Ramavathu and J. N. Mude. 2016. Acute toxicity of mercuric chloride (HgCl2) on survival and behavioural responses of freshwater fish Labeo rohita (Hamilton). Int. J. fisheries Aquatic sci., 4:323-327.
  13. Naserabad, S., et al. 2015. Acute toxicity and behavioural changes of Caspian roach (Rutilus rutilus caspicus Jakowlew, 1870) exposed to the fungicide Hinosan. African J. Biotech., 14(20): 1737-1742.
  14. Harit, G. and N. Srivastava. 2018. Behavioural alterations in Channa punctatus after exposure to endosulphan followed by subsequent recovery. Int. j. Fisheries Aquatic Sci., 6:51-55.
  15. Dwivedi, S. and P. Trivedi 2015. Behavioural, morphological and chromatophoric alterations induced by arsenite exposure in freshwater fish Channa punctatus (Bloch.). Int. J. Pharma Biosci., 6(3):1328-1334.
  16. Kapil, S.R., et al. 2008. Effects of cypermethrin on chromatophores of freshwater fish Channa punctatus. Taal 2007: the 12th world lake Conference. Proceedings, pp 571-577. DOI: 10.1007/s00128-002-0071.
  17. Sharma, M. 2019. Behavioural responses in effect to chemical stress in fish: A review. Int. J. Fisheries Aquatic Studies. (7):1-5.
  18. Ebrahimpour, M., M. Mosavisefat and R. Mohabbati. 2010. Acute toxicity bioassay of mercuric chloride: An alien fish from a river. Toxicol. Env. chem., 92:169-173. DOI: 10.1080/0277224090 2794977.
  19. Singh, Zorawar., T. Kaur and M. Kaur. 2017. Toxicological assessment of barium carbonate on behavioural and hematological parameters in Channa punctatus. Int. j. Medical Res. Health Sci., 6(5): 88-95.
  20. Raj, V., et al. 2013. Acute toxicity bioassays of cadmium and mercury on the juveniles of Asian seabass lates calcarifer (Bloch). Indian J. Sci. Tech., 6:4329-4335.
  21. Allen, T., A. Awasthi and S.V.S. Rana. 2004. Fish chromatophores as biomarkers of arsenic exposure. Env. biol. fishes. 71:7-11.
  22. Kaur, R. and A. Dua. 2012. Fish scales as indicator of wastewater toxicity from an international water channel Tung Dhab drain. Env. monit. assess., 184:2729-2740. DOI: 10.1007/s10661-011-2147-y.
  23. Biswas, S.P., A. G. Jadhao and N. V. Palande. 2014. Role of catecholamines and nitric oxide on pigment displacement of the chromatophores of freshwater snakehead teleost fish, Channa punctatus. Fish physiol. Biochem., 40: 457-467.  DOI: 10.1007/s10695-013-9857-8.