IJEP 42(5): 559-564 : Vol. 42 Issue. 5 (May 2022)
1. National Agronomic Research Institute of Algeria (INRAA), Setif Research Unit, Algeria
2. Setif 1 University, Faculty of Sciences of Life and Nature, Department of Biology and Vegetal Ecology,
3. Setif 1 University, Faculty of Sciences of Life and Nature, Department of Animal Biology and Physiology, Algeria
The objectives of this study are to quantify the imidacloprid pesticide residues in cucumber samples; to study the in-vitro effects of this pesticide on lipid peroxidation and hemolytic activity and finally, based on the mathematical models we estimate the values equivalent to the concentrations of pesticide residues detected in the vegetable samples. For the extraction of pesticide residues from vegetable samples we used the FaPEx kits. Agilent Technologies 1260 infinity high performance liquid chromatography having UV/visible detector was used for the identification and quantification of pesticides residues. The results of this study proved the presence of pesticide residues in cucumber tested with higher values compared with their corresponding MRLs. The in-vitro study of the effects of the imidacloprid pesticide on lipid peroxidation and hemolytic activity demonstrate significant effects of the different concentrations on both activities. The estimation by mathematical models of malondialdehyde quantity and hemolytic activity equivalent to the concentrations of the pesticide residues detected in vegetable samples proved the non-toxicity effects of these residues.
Imidacloprid, Residues, In-vitro, Lipid peroxidation, Hemolytic activity
- Duzguner, V. and S. Erdogan. 2012. Chronic exposure to imidacloprid induces inflammation and oxidative stress in the liver and central nervous system of rats. Pesticide Biochem. Physiol., 104:58-64.
- Kamrin, M.A. 1997. Pesticide profiles : Toxicity, environmental impact and fate. CRC Press.
- D‘Almeida, V.H., et al. 1997. Absence of oxidative stress following paradoxical sleep deprivation in rats. Neurosci. Lett., 235:25-28.
- Kanbur, M., et al. 2008. Effects of cypermethrin, propetamphos and combination involving cypermethrin and propetamphos on lipid peroxidation in mice. Env. Toxicol., 23(4):473-479.
- Duzguner, V. and S. Erdogan. 2010. Acute oxidant and inflammatory effects of imidacloprid on the mammalian central nervous system and liver in rats. Pestic. Biochem. Physiol., 97:13-18.
- Wen, Y., et al. 2009. Imidacloprid resistance and its mechanisms in field populations of brown planthopper Nilaparavata lugens stal in China. Pest. Biochem. Physiol., 94(1):36-42.
- Datta, C., et al. 2007. Effect of organophosphorus insecticide phsphomidon on antioxidant defence components of human erythrocyte and plasma. Indian J. Exp. Biol., 30:65-67.
- Daraghmeh, A., et al. 2007. Imidacloprid residues in fruits, vegetables and water samples from Palestine. Env. Geochem. Health. 29(1):45-50.
- Beleguet, B., et al. 2020. Assessment of human health risk associated with the imidacloprid pesticide. Indian J. Env. Prot., 40(2):155-160.
- Chuang, W.C., et al. 2019. FaPExÒ multipesticide residues extraction kit for minimizing sample preparation time in agricultural produce. J. AOAC Int., 102:1864-1876.
- Ohkawa, H., N. Ohishi and K. Yagi. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 95(2):351-358.
- Janero, D.R. 1990. Malondialdehyde and thiobarbituric acid reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radical Biol. Medicine. 95(6):515-540.
- Amin, K. and R.M. Dannenfelser. 2006. In-vitro hemolysis : Guidance for the pharmaceutical scientist. J. Pharm. Sci., 95:1173-1176.
- Banerjee, B.D., et al. 1999. Biochemical effects of some pesticides on lipid peroxidation and free radical scavengers. Toxicol. Lett., 107:33-47.
- Ruas, C.B., et al. 2008. Oxidative stress biomarkers of exposure in the blood of cichlid species from a metal-contaminated river. Ecotoxicol. Env. Saf., 71:86-93.
- Abdallah, F., et al. 2012. Caffeic acid and quercetin protect erythrocytes against the oxidative stress and the genotoxic effects of lambda-cyhalothrin in-vitro. Hum. Exp. Toxicol., 31:92-100.
- Fetoui, H., et al. 2008. Oxidative stress induced by lambda-cyhalothrin in rat erythrocytes and brain: Attenuation by vitamin C. Env. Toxicol. Pharmacol., 26:225-231.
- Bukowska, B. and S. Kowalska. 2004. Phenol and catechol induce prehemolytic and hemolytic changes in human erythrocytes. Toxicol. Lett., 152:73-84.
- Singh, M., R. Sandhir and R. Kiran. 2004. In-vitro effects of organophosphate pesticides on rat erythrocytes. Indian J. Exp. Biol., 42:292-296.
- Mohany, M., B.G. Rafaat and M. El-Feki. 2011. Immunological and histological effects of exposure to imidacloprid insecticide in male albino rats. African J. Pharm. Pharmacol., 5:2106-2114.
- Kapoor, U., et al. 2010. Effect of imidacloprid on antioxidant enzymes and lipid peroxidation in female rats to derive its no observed effect level (NOEL). J. Toxicol. Sci., 35:577-581.
- Chihuailaf, R.H., P.A. Contreras and F.G. Wittwer. 2002. Pathogenesis of oxidative stress : Consequences and evaluation in animal health. Vet. Mex., 33:265-283.
- Narendra, M., et al. 2007. Prallethrin induced biochemical changes in erythrocyte membrane and red cell osmotic haemolysis in human volunteers. Chemosphere. 67:1065-1071.
- Uchendu, C., et al. 2014. Erythrocyte osmotic fragility and the lipid peroxidation following chronic co-exposure of rats to chlorpyrifos and deltamethrin and the beneficial effect of alpha-lipoic acid. Toxicol. Reports. 1:373-378.