Imidacloprid Pesticide residues: The estimation of Lipid peroxidation and Osmotic Fragility effects

IJEP 42(5): 559-564 : Vol. 42 Issue. 5 (May 2022)

Ali Guendouz1*, Assia Belguet2 and Saliha Dahamna3

1. National Agronomic Research Institute of Algeria (INRAA), Setif Research Unit, Algeria
2. Setif 1 University, Faculty of Sciences of Life and Nature, Department of Biology and Vegetal Ecology,
3. Setif 1 University, Faculty of Sciences of Life and Nature, Department of Animal Biology and Physiology, Algeria


The objectives of this study are to quantify the imidacloprid pesticide residues in cucumber samples; to study the in-vitro effects of this pesticide on lipid peroxidation and hemolytic activity and finally, based on the mathematical models we estimate the values equivalent to the concentrations of pesticide residues detected in the vegetable samples. For the extraction of pesticide residues from vegetable samples we used the FaPEx kits. Agilent Technologies 1260 infinity high performance liquid chromatography having UV/visible detector was used for the identification and quantification of pesticides residues. The results of this study proved the presence of pesticide residues in cucumber tested with higher values compared with their corresponding MRLs. The in-vitro study of the effects of the imidacloprid pesticide on lipid peroxidation and hemolytic activity demonstrate significant effects of the different concentrations on both activities. The estimation by mathematical models of malondialdehyde quantity and hemolytic activity equivalent to the concentrations of the pesticide residues detected in vegetable samples proved the non-toxicity effects of these residues.


Imidacloprid, Residues, In-vitro, Lipid peroxidation, Hemolytic activity


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