Green Synthesized Silver Nanoparticles Assisted Heavy Metals Remediation Employing Bacillus cereus HD3PAH

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IJEP 43(9): 783-789 : Vol. 43 Issue. 9 (September 2023)

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Priya Borah, Arpita Borah, Paramita Chakravarty and Hemen Deka*

Gauhati University, Department of Botany, Guwahati – 781 014, Assam, India

Abstract

Two bacterial candidates, namely Paenibacillus sp. HD1PAH and Bacillus cereus HD3PAH; isolated from the crude oil-contaminated soil, were used for removal of Zn, Mn and Cu in liquid culture environments. The Paenibacillus sp. HD1PAH was used for synthesis of silver nanoparticles (Ag-NPs) whereas Bacillus cereus HD3PAH was employed for removal of Zn, Mn and Cu from liquid culture media. Three treatments were employed during 7 days of experimental trials; one with Bacillus cereus HD3PAH supplemented by silver nanoparticles (Ag-NPs), whereas in the other two, Bacillus cereus HD3PAH and Ag-NPs were employed alone. The results showed maximum reduction in Zn (94.75%), Mn (91.93%) and Cu (91.7%) in Bacillus cereus HD3PAH assisted with Ag-NPs employed treatment. Significantly, the time behavioural growth pattern of bacterial cells revealed a typical diauxic growth curve in presence of Ag-NPs and heavy metals (HMs) indicating the abilities of the bacterium to overcome the stress imposed due to Ag-NPs and HMs. Moreover, results also confirmed Paenibacillus sp. HD1PAH is the suitable source for synthesis of Ag-NPs indicating a size range of 58.7-71.8 nm under DLS alongwith the UV-visible and FTIR spectrum at around 200-400 nm and 2937.347/cm, 2112.531/cm and 1655.632/cm, respectively.

Keywords

Metal contamination, Bio-removal, Silver nanoparticles, Tolerance, Liquid culture

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